Lymph Node
Germinal center B cells
CD45+; CD20+; CD79a+ (weak); CD5-; CD10+; Ig+ (IgG, IgM or IgA, but not IgD); Light chain+; bcl-2-; bcl-6+; proliferating cases in dark zone well highlighted by Ki67
Mantle zone B cells:
CD45+; CD20+; CD79a+; IgD+; IgM+; IgG-; IgA-; Light chain+; bcl-2+; CD5-; CD10-; bcl-6-
Marginal zone B cells and monocytoid B cells
CD45+; CD20+; CD79a+; IgM+; IgD-/+; CD5-; bcl-2-; bcl-6-
Interfollicular B cells
CD45+; CD20+; CD79a+; Ig+ (IgD, IgM, IgG or IgA); Light chain+; bcl-2+; bcl-6-; activated B cells may express CD30; EBV-infected B cell may upregulate CD43
Plasma cells
CD45-/+; CD20-; CD79a+/-; Ig+; CD138+; EMA+/-
T cells
CD3+; CD45RO+; CD43+; mostly TCR-??, sometimes TCR-??; CD4+ or CD8+; subpopulation can express cytolytic molecules (granzyme B, TIA1, perforin)
T cells in germinal center
CD3+; CD45RO+; CD43+; CD57+; CD4+;CD8-
NK cells:
CD45+; surface CD3-; cytoplasmic CD3+; CD43+; CD45RO+; CD56+; CD57+/-; Granzyme B+; TIA1+; Perforin+
Follicular dendritic cells
CD45-; CD21+; CD35+; FDC+; CD23 (subpopulation)+; S100-/+; CD1a-; lysozyme-; HLA-DR+
Interdigitating dendritic cells
CD45+; S100+; CD68-/+; CD1a-/+; lysozyme-/+; HLA-DR+; CD43+
Langerhans cells
CD45+; S100+; CD68-/+; CD1a+; lysozyme-/+; HLA-DR+
Histiocytes
CD45+; S100-; CD68+; CD1a-; lysozyme+; HLA-DR+; CD43+
Spleen Lymphocytes
Either B cells or T cells (see Lymph node)
Splenic sinus lining cells
Factor VIII+; CD31-/+; CD34-/+; CD8+; lysozyme+; CD68-/+
Splenic pulp cords
Rich in CD68+ cells
Thymus
Thymic epithelial cells
CK+ (esp. CAM5.2+, but 34?E12 shows up the epithelium of the medulla much better)
Thymic T lymphocytes in cortex
CD45+; CD3+; TdT+; CD1a+; CD99+; high Ki67 index
Thymic T lymphocytes in medulla
CD45+; CD3+; TdT-; CD1a-; CD99-
Thymic B lymphocytes in medulla
CD45+; CD20+ (sometimes with asteroid appearance); CD79a+
Skin and mucosal tissues and parenchymal organs
Epithelium
CK+ (glandular epithelium tends to express low molecular weight CK; and some epithelial cells may express specific CK subtypes); vimentin-/+; BerEP4+/-; EMA+/-; E-cadherin+/-
Special types of epithelium
* Skin epidermis
- 34?E12+, CAM5.2-, CK5/6+
* Prostatic acini and ducts
-- PSA+
* Breast with apocrine change
-- Brst2+
* Glandular epithelium
-- CEA may be positive (luminal); EMA often positive (esp. luminal)
* Pneumocytes
-- Surfactant/PE10+, TTF1+
* Liver cells
-- CK+; HEP-PAR1+; CAM5.2+; AE1/AE3-; polyclonal CEA or CD10 in canalicular pattern; albumin+
* Bile ducts
-- CK+;CAM5.2+; AE1/AE3+, HEP-PAR1-
Myoepithelium
CK+; CK14+; High molecular weight CK+; Actin+; calponin+; S100-/+; GFAP-/+; Vimentin+
Basal cells of prostate
CK+; CK14+; Actin-; S100-
Melanocytes
S100+; Vimentin+; HMB45- (some junctional melanocytes can be +); CK-; c-kit+
Merkel cells
CK+; CK20+; synaptophysin+; chromogranin+; NSE+; neurofilament+
Mesenchymal cells
Fibroblasts
Vimentin+; Actin-; Desmin-; CK-
Myofibroblasts
Vimentin+; Actin+; Desmin-/+; CK-/+
Smooth muscle
Vimentin+; Actin+; Desmin+; CK-/+
Interstitial dendritic cells
Vimentin+; CD34+; Actin-/+; Desmin-
Endothelium
Factor VIII related antigen+; CD31+; CD34+; Vimentin+
Pericytes
Factor VIII related antigen-; CD31-; CD34-; vimentin+; Actin+; Desmin-
Fat cells
Vimentin+; S100+
Cartilage cells
Vimentin+; S100+; GFAP+/-
Skeletal muscle
Vimentin+; Actin+/-; Desmin+; Myoglobin+; MyoD1- (positive only in primitive or fetal muscle cells); Myogenin- (positive only in primitive or fetal muscle cells); S100-/+ (weak staining); CD56-/+ (positive in damaged muscle)
Endometrial stromal cells
CK-; Vimentin+; Actin+/-; Desmin-/+; S100-; CD10+
Mesothelial cells
Mesothelial cells
CK+; CK5/6+; Vimentin+; Calretinin+; HBME1+; N-cadherin+; E-cadherin-; CEA-; BerEP4-; LeuM1-; Desmin -/+ (occasional)
Neuroendocrine and endocrine organsThyroid follicular cells
CK+; Thyroglobulin+; TTF1+; Chromogranin-; Synaptophysin-; NSE-
Thyroid C cells
CK+; Calcitonin+; TTF1+; Chromogranin+; Synaptophysin+; NSE+; CEA+
Parathyroid
CK+; Parathyroid hormone+; TTF1-; Chromogranin+;Synaptophysin+; NSE+; Neurofilament+
Islet cells
CK+; Synaptophysin+; Chromogranin+; NSE+; Various hormones+
Pituitary cells
CK+; Synaptophysin+; Chromogranin+; NSE+; Various hormones+
Adrenal cortical cells
CK+/-; Synaptophysin-; Chromogranin-; NSE-; melan-A+/-; inhibin+/-
Dispersed neuroendocrine cells
CK+; Synaptophysin+; Chromogranin+; NSE+; Various hormones+
Central and peripheral nervous system
Neurons and ganglion cells
CK-; Neurofilament+; NSE+; Synaptophysin+; Chromogranin-/+; Calretinin+/-; GFAP-
Nerve fibers (CNS or peripheral)
CK-; Neurofilament+; NSE+; Synaptophysin+; Chromogranin-/+; GFAP-; Leu7+
Astrocytes
CK-, GFAP+; S100+; Vimentin+; Synaptophysin-; Neurofilament-
Oligodendroglial cells
CK-, GFAP-; S100+; Leu7+/-; Synaptophysin-; Neurofilament-
Ependymal cells
CK-/+; GFAP+; Vimentin+; EMA+/-
Choroid plexus cells
CK+; Transthyretin+; GFAP+/-; BerEP4-; CEA-; S100+/-
Schwann cells
CK-, S100+; GFAP-/+; Vimentin+; Neurofilament-; EMA-
Perineurial cells and meningeal cells
CK-, S100-; GFAP-; EMA+; Vimentin+
Paraganglion chief cells and adrenal medulla cells
CK-; Synaptophysin+; Chromogranin+; NSE+; Neurofilament+; S100-
Sustentacular cells
CK-, S100+; GFAP-/+; Synaptophysin-; Neurofilament+
Gonads
Sertoli cells
CK+/-; CD99+; Inhibin+; CD56-/+
Leydig cells
CK-; Synaptophysin-; CD56-/+; Inhibin+; Melan A+/-
Granulosa cells
CK-; Vimentin+; CD99+; Inhibin+; Melan A+/-
Germ cell (spermatogonia, ovum)
CK-; PLAP-; c-kit+
Placenta
Syncytiotrophoblast
CK+; HCG+
Cytotrophoblast
CK+; HCG-
Intermediate trophoblast
CK+; HCG-/+; HPL+
Decidual cells
CK-; Vimentin+
Bone marrow
Granulocytic series cells
CD45+; Myeloperoxidase+; KP1/CD68+; PGM1/CD68-; lysozyme+
Monocytic series cells
CD45+; Myeloperoxidase-; KP1/CD68+; PGM1/CD68+; lysozyme+
Red cell series
Glycophorin A+
Megakaryocytes and platelets
Factor VIII-related antigen+; CD31+; CD34+
